Download Anwendung von RFID-Systemen, 2.Auflage German by Christian Kern PDF

By Christian Kern

Die Radio-Frequenz-Identifikation (RFID) erm?glicht den drahtlosen Informationsaustausch zwischen Objekten, Personen, Tieren und dem IT-Netzwerk. Objekte, Personen oder Tiere werden dabei selbst zu Datentr?gern. Leser werden hier in die Lage versetzt, eine RFID-Anwendung von der Idee bis zur Praxis aufzubauen. Wie aktuell das Thema und entsprechend gro? die Nachfrage ist, zeigt das Erscheinen der 2. Auflage nach nur einem Jahr.

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Sterilize by autoclaving. 01 vol of sterile 20% glucose before use. 13. 100X Concentrate of appropriate antibiotic (see Note 2): Ampicillin is generally used at a final concentration of 100 pg/mL, and so the concentration for the 100X stock is 10 mg/mL. 5 mg/mL. 14. Ethanol (store at -20°C). 15. 0: Adjust the pH with acetic acid before making to the final volume. Filter and autoclave this solution and store at room temperature. 16. 0. 17. 70% Ethanol (store at -2OOC). 18. 0, 1 r&f EDTA. Filter and autoclave.

The tray should be filled with 10X SSC to 1 cm below the top of the sponge. Place two pieces of Whatman 3MM paper on the sponge and allow them to soak up the SSC. 8. , Wilmslow, UK) so that the buffer can only move through the gel. 9. Place the prewetted membrane on the gel, carefully avoiding trapping any air bubbles between the gel and the membrane. 10. Place six pieces of 3MM paper, cut to the size of the gel and prewetted in 10X SSC, on the membrane, again avoiding air bubbles. 11. Stack around 8 cm of paper towels on the 3MM paper, followed by a glass plate and a weight, such as a 500~mL bottle filled with water (suitable for a 15 x 10 cm gel) to compress the towels.

4. Column wash buffer: 1X column loading buffer. 5. 05% SDS. 1. Preparation 3. Methods of Total RNA from Solid Tissues 1, Freshly excised tissue should be frozen in liquid nitrogen and stored at -7OOC prior to use or used immediately. Using a Waring blender, or similar, that has been rinsed in DEPC-treated distilled water, break the tissue into small pieces in 6 mL of homogenization solution. Rinse the apparatus with 3 mL of homogenization solution and add to the 6 mL homogenate. Rinse the apparatus in DEPC-treated distilled water between each sample (see Note 3).

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